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Isolation and Analysis of Double-Stranded RNA from Virus-Infected Plant and Fungal Tissue

588
Citations
August 1, 2025
Published Date

Research Abstract & Technology Focus

A simple, rapid method for the isolation of double-stranded RNA (dsRNA) from virus-infected plant and fungal tissues provides a new approach to virus detection and identification. Diseased tissue was phenol-extracted to isolate cellular nucleic acids, and viral dsRNA was selectively purified from other nucleic acids by binding to cellulose powder in 15% ethanol either in small columns or by a batch procedure. The product was analyzed first by gel electrophoresis and then by ribonuclease treatment to identify dsRNA. The method permits rapid and efficient isolation and analysis of dsRNA from small amounts (1–10 g) of tissue and from multiple samples using small amounts (0.1–2.5 g) of cellulose powder. Successful isolation of dsRNA does not depend on the type of tissue processed and the method therefore is potentially useful for the study of RNA virus replication and for detection and diagnosis of virus infections directly from the infected host tissues.
isolation analysis double-stranded virus-infected plant fungal
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Isolation and Analysis of Double-Stranded RNA from Virus-Infected Plant and Fungal Tissue

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What is the core focus of the research titled 'Isolation and Analysis of Double-Stranded RNA from Virus-Infected Plant and Fungal Tissue'?

This literature focuses on: A simple, rapid method for the isolation of double-stranded RNA (dsRNA) from virus-infected plant and fungal tissues provides a new approach to virus detection and identification. Diseased tissue was phenol-extracted to isolate cellular nucleic ac...

Are there open-source GitHub repositories related to Isolation and Analysis of Double-Stranded RNA from Virus-Infected Plant and Fungal Tissue?

Yes, open-source projects like Mouseww/anything-analyzer (全能协议分析工具：浏览器抓包 + MITM 代理 + 指纹伪装 + AI 分析 + MCP Server 无缝对接 AI Agent/IDE | All-in-one protocol analysis toolkit — built-...) are actively building upon these concepts.

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What other academic literature is closely related to 'Isolation and Analysis of Double-Stranded RNA from Virus-Infected Plant and Fungal Tissue'?

Yes, highly correlated activity was mapped. An entry titled 'Isolation and Analysis of Double-Stranded RNA from Virus-Infected Plant and Fungal Tissue' discusses this: A simple, rapid method for the isolation of double-stranded RNA (dsRNA) from virus-infected plant and fungal tissues provides a new approach to vir...

Are there commercial applications of 'Isolation and Analysis of Double-Stranded RNA from Virus-Infected Plant and Fungal Tissue' in market news publications?

Yes, highly correlated activity was mapped. An entry titled 'In vivo site-specific engineering to reprogram T cells' discusses this: Stable and cell-specific transgene expression can be achieved through in vivo site-specific integration of large DNA payloads using a two-vector sy...

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